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Nairaland Forum / Nairaland / General / Health / N-terminal Sequence Analysis (244 Views)
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N-terminal Sequence Analysis by mytilo: 7:00am On Sep 23, 2016 |
Glycosylation, the attachment of sugar moieties to proteins, is a post-translational modification (PTM) that provides greater proteomic diversity than other PTMs. n-terminal sequence analysis Glycosylation is critical for a wide range of biological processes, including cell attachment to the extracellular matrix and protein-ligand interactions in the cell. This PTM is characterized by various glycosidic linkages, including N-, O- and C-linked glycosylation, glypiation (GPI anchor attachment) and phosphoglycosylation. Glycoproteins can be detected, purified and analyzed by different strategies, including glycan staining and visualization, glycan cross-linking to agarose or magnetic resin for labeling or purification, or proteomic analysis by mass spectrometry, respectively. |
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