Protein molecules play a key role in kit development, diagnostic reagent development, and drug discovery. The primary structure of the protein molecule itself is the core of its biological function. Therefore, the accurate and rapid analysis of the complete sequence of the protein contributes to the functional study of the unknown protein, and is significant to the development and production of protein drugs including monoclonal antibodies.

Traditional protein sequence analysis technology, whether based on MALDI-TOF mass spectrometer or nanoLC-MS/MS platform, requires the use of a sequence database containing the identified proteins in the protein identification process, and then the molecular weight data measured by mass spectrometry can be compared to the molecular weight data obtained after fragmentation of the database theoretical sequence, so as to achieve sequencing and identification of the protein.